Microbial Ecology Lab Protocols
A collection of bench-ready protocols for soil microbial ecology research. Each protocol includes an interactive reagent calculator that scales volumes to your sample count, a step-by-step checklist you can tick off at the bench, and a bench record template to fill in during the run.
Protocols are written for environmental soil samples and cover RNA/DNA extraction, DNase treatment, cDNA synthesis, and qPCR workflows.
Bench Manuals
Step-by-step bench references with interactive checklists and reagent calculators.
| Manual | Kit | Elapsed |
|---|---|---|
| Soil DNA Extraction (96-well) | DNeasy 96 PowerSoil Pro | ~2–3 h |
| Soil RNA Extraction | RNeasy PowerSoil Total RNA | ~2.5–3.5 h |
| Soil RNA DNase Removal & cDNA Synthesis | TURBO DNA-free · SuperScript IV | ~2.5–4 h |
| Agarose Gel Electrophoresis | GelGreen · GelRed | ~25–60 min |
| Regular PCR | PCR master mix | ~1.5–2.5 h |
| Ammonium and Nitrate Colorimetry Assay | 1M KCl extraction · SpectraMax ABS Plus | ~1–2 d |
Full Protocols (comprehensive workflow documents) coming soon.